Sequences of the complete nuclear ribosomal dna rdna gene from five individual paramphistomum cervi were determined for the first time. Various host animals are often infected concurrently with p. This study was undertaken to identify paramphistomum cervi on the basis of its morphology and histology to be. Electrophoretic analysis of various developmental stage specific somatic antigens of paramphistomum epiclitum digenea. Animal diversity web of michigan university the merck veterinary manual springer. The livestock rumen fluke, paramphistomum cervi, is found in mostly tropical and subtropical regions, including australia, asia, africa, eastern europe, and russia. It was concluded that paramphistomum cervi is prevalent in central punjab. Pdf identification and prevalence of paramphistomum cervi in. The following is an archived discussion of paramphistomum, paramphistomum cervi s dyk nomination. The present study records the occurrence of major lipid fractions and their fatty acids in a digenetic trematode parasite paramphistomum cervi, and the rumen fluid and liver of the goat capra hircus. Morphological and molecular characterization of paramphistomum.
The humoral igg response generated in goats experimentally infected with metacercariae of p. Prevalence and risk factors associated with fasciola. Pdf characterization of the complete nuclear ribosomal dna. Adult paramphistomum cervi or rumen fluke are pearshaped, slightly concave ventrally and convex dorsally. The whole life cycle of paramphistomum microbothrium fischoeder, 1901, found in cattle of kenya, east africa, has been established experimentally in laboratory conditions, the eggs hatched miracidia on the 14th to 16th day, if they were kept in water at a temperature of 2628 c. Evaluation of antibody response to various developmental. Adult flukes are located in the rumen of ruminants and immature flukes in the small intestine mainly in the duodenum 1,10. The worm measures about 5 mm in length and 25 mm in width across the midsection. The objective of this report was to investigate the effects of pb on motility, survival and tegument structure of adult p. They are responsible for the serious disease called paramphistomiasis, also known as amphistomosis, especially in cattle and sheep. Prevalence and pathological study of paramphistomum infection in the small intestine of slaughtered ovine. Uniquely, unlike most parasites, the adult worms are relatively harmless, but it is the developing juveniles that cause serious disease called paramphistomiasis or classically. Characterization of the complete nuclear ribosomal dna.
In order to address this emerging threat to ruminant farming systems, and associated risks for food security posed by rumen fluke infection, it is imperative that we develop a better. Pdf prevalence of paramphistomum cervi in ruminants. These are essentially rumen flukes, of which paramphistomum cervi is the most notorious in terms of prevalence and pathogenicity. Uniquely, unlike most parasites, the adult worms are relatively harmless, but it is the developing juveniles that cause serious disease called paramphistomiasis or classically amphistomosis. Pdf prevalence of paramphistomum daubneyi infection in. Research open access mitochondrial and nuclear ribosomal dna. Pdf identification and prevalence of paramphistomum. Abstract gastrointestinal trematode parasites especially paramphistomum cervi are accountable for health hazards in buffalo of central punjab. Prevalence and pathological study of paramphistomum.
Prevalence of paramphistomum cervi in ruminants slaughtered in district muzaffar garh article pdf available in pakistan veterinary journal 294 january 2009 with 844 reads how we measure reads. Evaluation of anthelmintic activity of ethanolic extracts of. Dec 26, 2016 the two ruminant parasites, paramphistomum cervi and carmyerius gregarius, were collected from freshslaughtered native cattle at local abattoirs in sadat district, menoufia province and identified morphologically, then molecularly by sequencing the nucleotides of 18s ribosomal rna gene 18s rrna. In the present study, the complete mitochondrial mt genome of p. Annual maturation of paramphistomum cervi trematoda. The prevalence of paramphistomum cervi infestation in balochi, babrik and harnai sheep breeds from balochistan pakistan was studied during september to december 2010. At least 14 species have been described in domestic ruminants of which the most important. Paramphistomum cervi, the type species of paramphistomum, is a parasitic flat worm belonging. C this article has been rated as cclass on the projects quality scale. Whilst historically regarded as being of minor importance in european livestock, recent evidence suggests that the prevalence of paramphistomosis is greater than that of fasciolosis in parts of the uk. Morphology of the flukes had described the features of paramphistomum genus. Silverstained protein profiles from whole worm extracts of adult paramphistomum cervi separated by sdspage, and immunoblot analysis of these proteins using infected cattle sera as probes. Prevalence of paramphistomum infection in cattle and sheep in. The adult fluke is plump and cylindrical unusual for a trematode.
The common rumen fluke, paramphistomum cervi is considered to be one of the most important species of paramphistomes 2. The mean intensity of infection in brahman breed was higher 652. On the species of paramphistomum fischoeder, 1901, occurring in britain and. This study determine the prevalence and risk factors associated with fasciola and paramphistomum and the coinfection of both parasites in 246 philippine carabaos slaughtered in ormoc city abattoir. We determined the complete mitochondrial dna mtdna sequence of a fluke, paramphistomum cervi digenea. The amount of neutral lipids nl, glycolipids gl and phospholipids pl of goat liver, rumen fluid and of the parasite shows that the rumen fluid is rich in nl, which is also in maximum. Characterization of the complete nuclear ribosomal dna sequences of paramphistomum cervi. Jan 30, 2015 the common rumen fluke, paramphistomum cervi is considered to be one of the most important species of paramphistomes. If the inline pdf is not rendering correctly, you can download the pdf file here. Amphistomiasis in farm and wild mammals is due to infection of paramphistomes, such as the species of paramphistomum, calicophoron, cotylophoron, pseudophisthodiscus, etc. Research article characterization of the complete nuclear. Most flukes from moose killed during winter novembermarch were small and immature, whereas almost all were gravid from moose killed during summer julyaugust.
Paramphistomidae, namely, metacercariae mcag, immature intestinal flukes imiag, immature ruminal flukes imrag, and adult flukes aag, was done by native polyacrylamide gel electrophoresis. Paramphistomomes are rare in the uk, but can cause severe losses in ruminants in the wet tropics. Home about us subjects contacts advanced search help. The common rumen fluke, paramphistomum cervi is considered to be one of the most important species of paramphistomes. The development and morphology of the miracidium of. A variety of factors like age asanji and williams, 1987, sex and breed of the host pal and qayyum, 1992, grazing habits saifulislam et al. Abstract the two ruminant parasites, paramphistomum cervi and carmyerius gregarius, were collected from freshslaughtered native cattle at local abattoirs in sadat district, menoufia province and identified morphologically, then molecularly by sequencing the nucleotides of 18s ribosomal. Low this article has been rated as lowimportance on the projects importance scale. Prevalence and pathological study of paramphistomum infection. Paramphistomomes are rare in the uk, but can cause severe losses in ruminants in the wet tropics the adult fluke is plump and cylindrical unusual for a trematode. It is a tiny fluke mostly parasitising livestock ruminants, as well as some wild mammals.
Paramphistomum is a genus of parasitic flatworms belonging to the digenetic trematodes. The life cycle of paramphistomum microbothrium fischoeder. Conventional plantbased therapies act as an important therapeutic tool for the treatment of worm infections all over the world and continuous evaluation of medicinal plants to find new potential lead compounds should be carried out. International journal of current science and research rapid. These are essentially rumen flukes, of which paramphistomum cervi is the most notorious in. Paramphistomes are present worldwide, but the disease paramphistomosis is most common in the temperate and tropical climatic regions of australasia, africa, and asia. Unless there is consensus to reopen the archived discussion here.
Pdf morphological and histological identification of. Morphology and histology of the adult paramphistomum. Rumen flukes, presumably paramphistomum cervi schrank 1790, were found in the rumen of 49 of 60 moose 82% examined from 1976 to 1979. Descriptive studies of the genus paramphistomum fischoeder, 1901 in some nigerian cattle. Detection of paramphistomum cevi antibodies in rabbits.
Paramphistomum cervi antigenic profile adults as recognized by infected cattle sera. Eggs in feces were distinguished on the basis of their size. Paramphistomum cervi, trematode, buffalo, prevalence. Paramphistomum cervi, the type species of genus paramphistomum, is an parasitic flat worm belonging to the class trematoda. The small intestine of slaughtered sheep was collected weekly from abattoirs kermanshah, sanandaj, tabriz and urmia slaughterhouses to monitoring the seasonal occurrence of paramphistomosis, 2,421 sheep carcasses 743 male 30. Rumen flukes parasitize the rumen and reticulum of ruminants, causing paramphistomiasis. Upon postmortem examination, adult parasites were collected from the organ of localization and identified based on morphological characteristics. The nucleotide sequences of the two isolates were 456 p. More specifically, specimens have been reported in europe from bulgaria, italy, france, and poland and also in asia from thailand, india, and china. Paramiphistoma in the rumen of infected sheep in doaj. Morphological and histological identification of paramphistomum cervi. During the course of a recent study of bovine fascioliasis both p. Paramphistomum cervi is within the scope of wikiproject animals, an attempt to better organize information in articles related to animals and zoology.
Morphological and histological identification of paramphistomum. In the present study, we evaluated the histological morphology of the adult paramphistomum p. Antigenic profile of adults as recognized by infected cattle sera. Specificity of a coproantigen elisa test for fasciolosis. It attaches to the wall of the rumen without causing serious damage. A commercial coproantigen elisa test for fasciolosis, based on the use of mm3 monoclonal antibody for antigen capture, was investigated for possible crossreactivity with paramphistomum cervi, a trematode that commonly infects cattle and sheep grazing in flukeinfested pasture in ireland. A total of 289 rumens and reticulums of buffaloes were examined for the presence of paramphistomum cervi by. The identification of stained trematodes to the species revealed 40, 20, 20, 15 and 5% gastrothylax crumenifer, cotylophoron cotylophorom, paramphistomum cervi, carmyerius spatiosus, explanatum explanatum, respectively. Information about the openaccess article morphological and histological identification of paramphistomum cervi trematoda. This study was undertaken to identify paramphistomum cervi on the basis of its.
Aug 14, 2011 the purpose of this study is to determine the efficacies of hexane, chloroform, ethyl acetate, acetone, and methanol leaf extracts of euphorbia hirta l. Biomedical and pharmacology journal bpj is an international, open. The mitochondrial genome of paramphistomum cervi digenea, the. Mitochondrial and nuclear ribosomal dna dataset supports that. Pdf invitro anthelmintic activity of three bangladeshi. African journal of pharmacy and pharmacology evaluation. Prevalence of paramphistomum infection in cattle and sheep. Paramphistomiasis, a trematode infectious disease in ruminants, has been neglected but has recently emerged as an important cause of productivity loss.
Detection of paramphistomum cevi antibodies in rabbits using elisa and did. The mitochondrial genome of paramphistomum cervi digenea, the first representative for the family paramphistomidae. Paramphistomum leydeni and paramphistomum cervi are common rumen flukes in many countries 1, 2, particularly in argentina. Hepatic damage in buffalo infected with paramphistomum cervi is described here based on the study of histological sections of infected liver of the animal. This study was undertaken to identify paramphistomum cervi on the basis of its morphology and histology to be the common cause of paramphistomosis in infected sheep and its differentiation from other similar paramphistomes in gujarat. Paramphistomum cervi, the type species of paramphistomum, is a parasitic flat worm belonging to the class trematoda. Pdf first occurrence of paramphistomum microbothrium. Subsequent comments should be made on the appropriate discussion page.
Research open access mitochondrial and nuclear ribosomal. This is mainly applied for the determination of time of death d and time of paralysis p of the. Af the dorsal surface of adult paramphistomum cervi appears highly corrugated with major folds fo and major grooves gr as well as numerous microvilli mi on the microfolds covering the surface of anterior a and b, middle c and d and posterior e and f regions, while spines are absent. First occurrence of paramphistomum microbothrium fischoeder. The five complete rdna sequences, which included the 18s rdna, the internal transcribed spacer 1 its1, the 5. Major lipids and fatty acids in the liver and rumen fluid. Molecular identification of the rumen flukes paramphistomum. This genome 14,014 bp is slightly larger than that of clonorchis sinensis,875 bp, but smaller than those of other digenean species. International journal of current science and research. Prevalence of paramphistomum cervi in ruminants slaughtered in district muzaffar garh. Histological sections of adult and immature fasciola hepatica and p cervi were. Longitudinal and transversal serialsections were stained with hematoxylin and eosin, and examined.
Paramphistomum ichikawai and calicophoron calicophorum are present in australia, and paramphistomum cervi in france, italy, bulgaria, poland, india, and china. Histological sections of adult and immature fasciola hepatica and p cervi were incubated with mm3. Over the years, there has been considerable debate as to whether paramphistomum leydeni and paramphistomum cervi are the same or distant species. Pdf characterization of the complete nuclear ribosomal.
Preliminary molecular identification of two helminthes. Pdf descriptive studies of the genus paramphistomum. It includes flukes which are mostly parasitising livestock ruminants, as well as some wild mammals. First occurrence of paramphistomum microbothrium fischoeder 1901 in roe deer capreolus capreolus in serbia. Adult flukes with bodies 515 mm in length and 27 mm in width were subjected to histological analysis. Body was conical with concave ventral and convex dorsal surface, tegumental spines all around the body in the immature stage, terminal funnel shape oral.
Publish original research work of multidisciplinary field of science. Theseparasitesmay surviveuptoyearsprovidingavirtuallyconstantsourceofinfestation. Morphological and molecular identification can pave the way to design the most effective control measures against the paramphistomum epiclitum in small ruminants. A marked variation in antibody response was recorded with various antigens. Research article characterization of the complete nuclear ribosomal dna sequences of paramphistomum cervi xuzheng, 1 qiaochengchang, 1 yanzhang, 1 siqintian, 1 yanlou, 1 hongduan, 1 donghuiguo, 1 chunrenwang, 1 andxingquanzhu 1,2 college of animal science and veterinary medicine, heilongjiang bayi agricultural university, daqing. Maturation of paramphistomum cervi in sheep in india. As observed by scanning electron microscopy sem, the tegumental surface in all part of the body, appears highly corrugated with transverse folds alternating with grooves and is spineless. Rumen fluke of reindeer is usually referred to as p.